Fachgruppe Nanostrukturierte Materialien    


Publikationen
 
Lehre
 Vorlesungen & Semin.
 Angebote f. Studenten

Forschung
 Themen
 Projekte
 Publikationen

Labore/Techniken
Anschrift
 Ausstattung
Under Construction
Under Construction
Röntgenbeugung

Mitarbeiter
 Übersicht
 offene Stellen

Links
 Universität Halle
 Institut für Physik
 IZM
 IFOX
 Andere Links

Institut f. Physik
FG Nanostrukturierte Materialien
Martin-Luther-Universitat
Halle-Wittenberg
Von-Danckelmann-Platz 3,
D-06120 Halle, Germany

Tel.:  +49 345 55 25321
Fax.: +49 345 55 27034

[Veröffentlichungen] [Patente] [Graduierungsarbeiten] [Berichte] [Poster]
Abstract

Esmaeilzadeh, Pegah; Köwitsch, Alexander; Heyroth, Frank; Schmidt, Georg; Fischer, Steffen; Richter, Katja; Groth, Thomas
Synthesis of thiolated polysaccharides for formation of polyelectrolyte multilayers with improved cellular adhesion
Carbohydrate Polymers 157 (2017-04-14 13:22:40), 1205
DOI: http://doi.org/10.1016/j.carbpol.2016.10.088


Intrinsic cross-linking is not only useful for increasing stability, but also for tailoring mechanical properties of polyelectrolyte multilayers (PEM) on implants and tissue engineering scaffolds. Here, a novel route for synthesizing thiolated chitosan (t-Chi) based on the application of 3,3?-dithiodipropionic acid was applied, while thiolated chondroitin sulfate (t-CS) was conjugated by 3,3?-dithiobis (propanoic hydrazide). Both products were subsequently reduced to obtain the free thiols. The thiol content, structural changes and degree of substitution were studied by UV–vis, FTIR, Raman and 1H NMR spectroscopy, respectively. Chi and CS can be used for PEM formation with the layer-by-layer method, due to the cationic nature of Chi at pH values below 5.0 and the anionic character of CS. Comparative studies on the formation of native Chi/CS versus t-Chi/t-CS PEM with surface plasmon resonance and ellipsometry revealed higher layer mass. We also found that the PEM composed of t-Chi/t-CS had superior cell adhesion properties for human keratinocytes in comparison to the native PEM.

http://www.sciencedirect.com/science/article/pii/S0144861716312541
Impressum Copyright ©  Center of Materials Science, Halle, Germany. All rights reserved.